Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis
نویسندگان
چکیده
منابع مشابه
Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis
During the transition from the inner cell mass (ICM) cells of blastocysts to pluripotent embryonic stem cells (ESCs) in vitro, a normal developmental program is replaced in cells that acquire a capacity for infinite self-renewal and pluripotency. We explored the underlying mechanism of this switch by using RNA-Seq transcriptome analysis at the resolution of single cells. We detected significant...
متن کاملI-42: Origins and Differentiation of Somatic Progenitors of The Mammalian Gonad Revealed by Single Cell RNA-Seq
Background - MaterialsAndMethods N;Results N;Conclusion N;
متن کاملMechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines.
BACKGROUND For clinical grade human embryonic stem cell (hESC) lines, a robust derivation system without any substances having animal origin would be required. We have gradually improved our hESC derivations. Human skin fibroblasts were used as feeder cells in derivation of all our 25 permanent fully characterized hESC lines. In the first four derivations, fetal calf serum was used as a supplem...
متن کاملDerivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks.
Sir, Most of the human embryonic stem cell (hESC) lines have been derived from the inner mass of blastocyst stage embryos, some from 8-cell stage morula (Streclchenko et al., 2004) and some from isolated blastomeres from morulae (Chung et al., 2008). In a very recent abstract, a Belgian team (Van de Velde et al., 2008) elegantly established an embryonic stem cell line from a single blastomere i...
متن کاملThe effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells
Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Stem Cell
سال: 2010
ISSN: 1934-5909
DOI: 10.1016/j.stem.2010.03.015